Structural analysis of DNA binding by C.Csp231I, a member of a novel class of R-M controller proteins regulating gene expression

Shevtsov, M.B.; Streeter, S.D.; Thresh, S.-J.; Swiderska, A.; McGeehan, J.E.; Kneale, G.G.

doi:10.1107/S139900471402690X

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 3
DNA-binding analysis. EMSAs showing the binding of C.Csp231I to various DNA sequences: (a) native 54 bp (54-1), (b) a 54-mer with a random second site (54-2), (c) a 54-mer with a random spacer (54-3) and (d) a 54 bp DNA fragment lacking the central 6 bp spacer (48-1). Precise sequences of the oligonucleotide duplexes are shown in Fig. 1

. DNA duplexes were incubated at protein (subunit):DNA molar ratios of 0, 1, 2, 4 and 8 in each case. The DNA concentration was maintained at 2 µM throughout.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 71| Part 2| February 2015| Pages 398-407

https://doi.org/10.1107/S139900471402690X

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