Figure 1
The structure of S. cerevisiae α-mannan and a potential model for its hydrolysis by Bt. Exo-acting GH92 α-mannosidases remove α-1,2- and α-1,3-linked side-chain moieties, whilst GH76 enzymes hydrolyse the α-1,6 backbone, allowing the import of smaller mono-oligosaccharides for further digestion. Carbohydrate residues are shown according to CFG (Centre for Functional Glycomics) nomenclature: green circles denote mannose, whilst blue squares represent N-acetylglucosamine. Linkages are specified. |