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![[Figure 4]](dz5353fig4mag.jpg)
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Figure 4
(a) Sequence alignment of the putative catalytic regions of the ten GH76 enzymes encoded by Bt, plus BcGH76 and lin0763 from L. innocua (the remaining GH76 enzymes for which structures are currently known). Identical residues are shown highlighted in red, whilst highly similar amino acids are highlighted in yellow. Within the consensus sequence (bottom row), full identity is depicted by uppercase letters, a consensus score of >0.5 is depicted by lowercase letters and amino acids with similar chemical properties are indicated via symbols (! is I or V, $ is L or M, % is F or Y and # is any one of N, D, Q or E). (b) Organization of genes within PUL 43 (as defined by Martens et al., 2008  ). Boxes are scaled relative to the size of the largest gene (BT2952, 2967 base pairs, 988 amino acids), with genes with known/assigned function shown in colour. The alignment in (a) was prepared using the PRALINE server (Heringa, 1999; Simossis & Heringa, 2005), with secondary-structure assignment using ESPript (Robert & Gouet, 2014). The three-dimensional structures of all known GH76 enzymes were included to inform the final alignment; however, for simplicity, only the secondary structure of BT2949 (the subject of this work) is shown. |
![[Figure 4]](dz5353fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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