|
Figure 4
Paramagnetic labels attached to the FnIII-3 and FnIII-4 domains of β4. (a) The predicted average locations of the nitroxide group of MTSL attached to Cys are shown as spheres in the crystal structure of FnIII-3. The positions that yielded well defined inter-spin distances with a second probe at C1608 are coloured green. Those that resulted in broad distance distributions but did not alter the global structure of FnIII-3,4 are coloured magenta. The positions that resulted in a distortion of the structure are coloured dark violet. (b) Crystal structure of FnIII-4 with the estimated average position of the paramagnetic groups. The positions that yielded useful distances are coloured green, while those that resulted in broad distributions are shown in pink. |