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Figure 6
DEER results for triply labelled FnIII-3,4 mutants. (a) Normalized dipolar evolution (black lines) and fits to the data (grey lines) for each of eight triply labelled mutants. (b) Distance distribution profiles calculated from the data in (a). The positions of peaks that correspond to distances previously observed in doubly labelled mutants containing C1608 are shown in rectangles. The positions of peaks assigned to intradomain distances modelled on the FnIII-4 structure are underlined. Finally, the peaks attributed to interdomain distances that do not involve C1608 are shown in grey circles. See Supplementary Fig. S10 for analysis of these proteins by SAXS.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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