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![[Figure 2]](mh5172fig2mag.jpg)
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Figure 2
Pressure-induced changes in the structure of HEWL with respect to the structure at 0.1 MPa: (a) 890 MPa, (b) 950 MPa. For each structure, the arrows indicate the displacement vectors, which show the shift in Cα positions between 0.1 MPa and the final pressure. For clarity, displacements larger than the threshold of 0.5 Å are drawn and the lengths of the displacement vectors are increased tenfold. The 890 MPa structure is distorted to close the active site and the distortions of molecules increase at 950 MPa. At 950 MPa, there are four molecules, A, B, C and D, in the asymmetric unit, and each is distorted differently as shown in this figure. The red colour in the cartoon model shows that the region has higher B factors than the average for each molecule. (c) Large motion detected between 0.1 MPa and molecule C at 950 MPa using DynDom. The cross indicates the centre of rotation. The domains of the structure at 0.1 MPa are shown in blue (Lys1–Thr40, Leu83–Leu129) and red (Thr43–Ser81) and the corresponding domains at 950 MPa are shown in purple and brown, respectively. The residues in green (Gln41–Ala42, Ala82–Leu83) provide the hinges for the movement. |
![[Figure 2]](mh5172fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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