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![[Figure 5]](mn5084fig5mag.jpg)
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Figure 5
The tetramer was confirmed to be the biological unit using a number of biophysical techniques. (a) Small-angle X-ray scattering data were compared with scattering data generated using CRYSOL (Svergun et al., 1995  ) for a monomer, a dimer and a tetramer, with the best fit for the tetramer, and a SAXS envelope was generated using the experimental data. (b) YpTesB eluted from a size-exclusion column consistent with a tetramer, as confirmed using a standard curve of the size-exclusion column (inset) to determine the elution volumes of a monomer, a dimer, a trimer and a tetramer (red). (c) The continuous mass [c(M)] distribution is plotted as a function of molecular mass (kDa) for TesB (2.4 mg ml−1). The molecular mass at the ordinate maximum of the peak shown corresponds to 120 kDa. The c(M) distribution was calculated using 200 masses from 0 to 300 kDa at a P-value of 0.95, which resulted in an r.m.s.d. of 0.00685 and a runs test Z of 7.61 and yielded a frictional ratio of 1.28. Inset: residuals for the c(M) best fit plotted as a function of radial position. |
![[Figure 5]](mn5084fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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