Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array

Lyubimov, A.Y.; Murray, T.D.; Koehl, A.; Araci, I.E.; Uervirojnangkoorn, M.; Zeldin, O.B.; Cohen, A.E.; Soltis, S.M.; Baxter, E.L.; Brewster, A.S.; Sauter, N.K.; Brunger, A.T.; Berger, J.M.

doi:10.1107/S1399004715002308

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 3
A microfluidic trap array for protein microcrystals. (a) Schematic representation of the crystal-capturing device design. (b) Close-up of the general scheme for trap-and-bypass hydrodynamic crystal capture. (c) Schematic of a single hydrodynamic trap [labeled in (b); W_T is the width of the trap channel and L_T is the length of the trap channel]. (d) Light micrograph of a representative section of a fabricated crystal-capture chip. (e) Light-micrograph series showing single and multiple HEWL microcrystals immobilized in hydrodynamic traps.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 71| Part 4| April 2015| Pages 928-940

https://doi.org/10.1107/S1399004715002308

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