Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array

Lyubimov, A.Y.; Murray, T.D.; Koehl, A.; Araci, I.E.; Uervirojnangkoorn, M.; Zeldin, O.B.; Cohen, A.E.; Soltis, S.M.; Baxter, E.L.; Brewster, A.S.; Sauter, N.K.; Brunger, A.T.; Berger, J.M.

doi:10.1107/S1399004715002308

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 7
Solution and refinement of a tetragonal HEWL structure. (a) Electron-density maps (2F_o − F_c at 1.0σ, blue mesh; F_o − F_c at 2.5σ, green mesh) obtained from molecular replacement using a polyalanine model of tetragonal HEWL. (b) Final refined 2F_o − F_c map (1.5σ, blue mesh) of tetragonal HEWL at 2.5 Å resolution. (c) Ramachandran diagram of all amino-acid residues in the asymmetric unit. (d) Superposition of the tetragonal HEWL structure determined from in-chip diffraction data (orange) versus 15 tetragonal HEWL structures randomly drawn from the Protein Data Bank (grey).

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 71| Part 4| April 2015| Pages 928-940

https://doi.org/10.1107/S1399004715002308

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