Figure 5
Electron-density map (2mFo − DFc) from reflections associated with PDB entry 4s1l
to 1.75 Å resolution (Ginn et al., 2015 ) associated with Trp163 (a) and Lys52 (d), their corresponding electron density in the initial merge for the higher resolution data set, (b) and (e), and the final presented 1.46 Å resolution structure, (c) and (f), at a σ of 1.5. The Lys52 side chain shows the prominence of the H atoms on the methylene groups, which are not pronounced on the perpendicular profile. The N, C and O atoms are distinguishable compared with the 1.75 Å resolution structure. The high-resolution information beyond 1.75 Å and post-refinement of this data set appear to have separate sequential improvements on the quality of the electron density compared with the 4s1l
structure. |