 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 3]](tz5076fig3mag.jpg)
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Figure 3
Crystal structure of HiTehA from in situ and cryogenic data. Cartoon representation of TehA (a) parallel to the membrane and (b) from the periplasmic face. The structure is coloured in a rainbow from the N-terminus (blue) to the C-terminus (red). (c) 2Fo − Fc electron-density map section within TM9 contoured at 1.0σ with the model in stick represention with carbon in yellow, nitrogen in blue and oxygen in red. Clear electron density is visible for the highly conserved gating residue Phe262. (d) 2Fo − Fc electron-density map for OG with its proximate residues from the in situ 2.3 Å resolution data contoured at 1σ. (e) The same representation as in (d) for the 1.5 Å resolution 100 K structure. (f) Ribbon representation with the OG detergent molecule and surrounding side chains shown as sticks. (g) A slice through the channel shows the path with the gating residue Phe262 (red sticks) on TM9. The OG detergent is bound to HiTehA on the cytoplasmic side and reaches deep into the hydrophobic channel. |
![[Figure 3]](tz5076fig3.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
