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Figure 4
Comparison of the in situ and cryogenic structures. (a) The two models superimpose quite well in general. One notable exception is the loop connecting TM6 and TM7, as detailed in (b). This shifting loop is located towards the adjacent monomer and is proximate to the C-terminal region. (c) Colour representation of B factor across the chain for the two models. The blue to red spectrum indicates low to high B factors. The structure obtained with the in situ data exhibits higher B factors, especially at the ends of the helices C-terminal to TM10, than the cryogenic structure. The respective overall B factors are 26.4 Å2 for the 295 K structure and 24.4 Å2 for the 100 K structure. On average, the intracellular part of the models has a higher B factor than the extracellular part owing to the presence of larger loops.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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