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Figure 2
Overall structure of NO66176–C complexed with Rpl8 peptide. (a) Cartoon representation of the crystal structure of NO66176–C complexed with Rpl8204–224, a peptide derived from Rpl8. The JmjC domain, the dimerization hinge domain, the β-hairpin motif and the C-terminal wHTH domain are shown in pink, cyan, orange and magenta, respectively. The Rpl8 peptide is coloured green. (b) The bound peptide Rpl8204–224 is shown as sticks. The Fo − Fc OMIT electron-density map for bound substrate was contoured at 3.0σ. A cartoon representation of the dimeric (c) and tetrameric (d) structure of NO66176–C in complex with Rpl8204–224 is shown. A dimer is present in the asymmetric unit. Two dimers related by crystallographic twofold symmetry pack against each other to form the tetramer. The filled oval indicates the twofold symmetry axis. |