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![[Figure 4]](tz5079fig4mag.jpg)
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Figure 4
Overall structure of ABP34. (a) Cartoon representation of the ABP34 monomer structure from front (left) and side (right) views. The molecule is coloured progressively from blue at the N-terminus to red at the C-terminus. Secondary structures including helices and sheets are labelled. The calcium ion is highlighted with a black sphere and the disordered region (257–269) is shown as a dotted line. (b) Structure-based multiple sequence alignment of ABP34 with the homologues from amoebal species. The secondary-structure elements of D. discoideum ABP34 are shown above the alignment (N-domain; blue, C-domain; red). Homologous regions are boxed, with identical amino-acid residues shown as bold white letters on a red background and functionally equivalent residues shown as red letters. Residues in the Ca2+-binding site are highlighted with black boxes and the conserved actin-binding residues are indicated by red dots. The proposed actin-binding sites ABS1, ABS2 and ABS3 are underlined in magenta and the proline kink is marked with an green asterisk and boxed in green. The full names of the strains are as follows: D. purpureum, Dictyostelium purpureum; D. fasciculatum, Dictyostelium fasciculatum; P. pallidum, Polysphondylium pallidum; P. polycephalum, Physarum polycephalum; A. castellanii, Acanthamoeba castellanii; E. histolytica, Entamoeba histolytica. |
![[Figure 4]](tz5079fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
