 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 5]](dw5145fig5mag.jpg)
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Figure 5
GemC1 interacts with Idas but does not interact with Cdt1. U2OS cells were co-transfected with vectors expressing GemC1-HA and Idas-GFP or Cdt1-GFP, as indicated. For each lane, following immunoprecipitation with anti-HA specific antibodies, total cell lysates and immunoprecipitates were analyzed by Western blotting with anti-GFP (upper panel) and anti-HA (lower panel) specific antibodies. In the lower panel, the grey star marks the large chain of the IgGs present in the anti-HA immunoprecipitates. The unlabelled bands in the upper panel are nonspecific bands for the weak interaction of GPA with Idas-GFP, but no interaction with Cdt1-GFP is detected. |
![[Figure 5]](dw5145fig5.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
