Figure 1
The MeshAndCollect workflow for a multicrystal data-collection method. (a) A mesh scan is performed on the sample. The resulting images are automatically inspected for protein diffraction and scored according to diffraction strength. A heat map is generated that represents the diffraction intensity, where the positions for partial data collections are marked. After the user has selected the settings for the partial data collections, the MxCuBE2 data-collection queue is automatically filled and all partial data sets are collected. Once the partial data sets have been automatically processed, HCA can then be used to choose which data sets to merge to produce a final data set for structure solution. (b) Flow diagram of the MeshAndCollect workflow used. |