 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 8]](wa5100fig8mag.jpg)
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Figure 8
Solution X-ray scattering studies of the NS5FL mutants d3-NS5-d4linker and d4-NS5-d3linker. (a) Schematic diagrams of the d3-NS5-d4linker and d4-NS5-d3linker mutant constructs. The linker of DENV-3 NS5 (263-HVNAEPETPN-272) was mutated to the linker of DENV-4 NS5 (264-SVSTETEKPD-273) and vice versa. (b) Pair-distance distribution functions P(r) for DENV-3 NS5FL (blue unbroken line), DENV-4 NS5FL (magenta unbroken line), d3-NS5-d4linker (blue dashed line) and d4-NS5-d3linker (magenta dashed line). (c) Normalized Krakty plots for lysozyme (grey filled circles), DENV-3 NS5FL (blue filled circles), DENV-4 NS5FL (magenta filled circles), d3-NS5-d4linker (blue circles) and d4-NS5-d3linker (magenta circles). The d3-NS5-d4linker mutant protein shows a bell-curve profile with a slight difference from that of wild-type DENV-3 NS5FL in the normalized Kratky plots, while the more bell-curved profile of d4-NS5-d3linker shifts inwards in the normalized Kratky plots when compared with those for wild-type DENV-3 NS5FL and DENV-4 NS5FL. This indicates that the linker of DENV-4 NS5FL is essential to preserve its compactness. |
![[Figure 8]](wa5100fig8.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
