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Figure 1
Site-directed mutagenesis disrupts crystal packing involving active-site surface loops. (a) Sequence alignment of P. aeruginosa DsbA1 with homologues from E. coli, K. pneumoniae and V. cholerae to identify a suitable nonpolar amino-acid substitution for Glu82 in P. aeruginosa (marked with an asterisk). The figure was generated using ESPript3 (Robert & Gouet, 2014 ![]() |