 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 1]](gm5040fig1mag.jpg)
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Figure 1
Site-directed mutagenesis disrupts crystal packing involving active-site surface loops. (a) Sequence alignment of P. aeruginosa DsbA1 with homologues from E. coli, K. pneumoniae and V. cholerae to identify a suitable nonpolar amino-acid substitution for Glu82 in P. aeruginosa (marked with an asterisk). The figure was generated using ESPript3 (Robert & Gouet, 2014  ). Comparison of symmetry-related molecule packing in crystals of WT PaDsbA1 (b) (blue) and E82I PaDsbA1 (c) (orange) indicates reduced involvement of the active-site surface loops (shown in red for each protein) in packing interactions in E82I PaDsbA1 relative to WT PaDsbA1. |
![[Figure 1]](gm5040fig1.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
