 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 1]](qh5032fig1mag.jpg)
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Figure 1
Size-exclusion chromatography of wild-type Sa_enolase. (a) Size-exclusion chromatography of Sa_enolase using a Superdex 200 16/60 column (GE). The molecular weights of the octamer and dimer peaks are calculated from the elution volume based on the standard curve. (b) Native PAGE analysis of Sa_enolase. The first lane (WT) is the sample before SEC; the second and third lanes correspond to the octamer and dimer peaks after SEC. Size-exclusion chromatographic analysis of the octameric (c) and dimeric (d) forms of Sa_enolase was performed using a Superdex 100 10/300 GL column (GE) to monitor the interconversion and equilibration of both forms. The chromatographic separation of the standard proteins is shown as a black dashed line and the theoretical molecular weights are shown above. |
![[Figure 1]](qh5032fig1.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
