Figure 7
EPR spectra of spin-labelled T43C on catalytic loop 1 of the Sa_enolase octamer and dimer. (a) Size-exclusion chromatography of the Sa_enolase T43C/C245S mutant using a Superdex 200 16/60 column (GE). (b) EPR spectra of the T43C/C245S dimer and octamer without 2-PG. EPR spectra of the T43C/C245S octamer (c) and dimer (d) in the presence or absence of 2-PG. Each spectrum was normalized by the height of the central peak. `i' and `m' represent the `immobile' and `mobile' components, respectively. |