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Figure 2
Biophysical characterization of MERS-CoV NTD+. (a) Binding of RNA to MERS-CoV NTD+ assessed by fluorescence polarization. 3′-Cy5-labelled RNA was incubated with various concentrations of NTD+. (b) MALLS/UV/refractometry/SEC analysis of NTD+. The right y axis represents the OD280 nm and the left y axis represents the molar mass in g mol−1 (Da). The absorption peak is shown as a noncontinuous line, with the continuous trace indicating the molar mass. The value of the measured mass at the volume corresponding to the top of the peak is reported.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 72| Part 2| January 2016| Pages 192-202
doi:10.1107/S2059798315024328
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