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Figure 4
Both protein and precipitant concentration increase very differently for different mixing ratios. Plotted here are the calculated concentrations at the start and the end of a vapour-diffusion experiment in which a hypothetical protein at 10 mg ml−1 is mixed with an example precipitant (sodium chloride) at 1 M at mixing ratios of 2:1, 1:1 and 1:2. These numbers capture the case that the protein does not change phase and that equilibration runs to completion, even though the rate of the process varies considerably depending on the chemical components (Luft & DeTitta, 1995 ![]() |