Figure 1
Change in absorbed dose as a function of energy for native and derivatived FcγRIII crystals. Dose calculations assume a beam size of 10 × 10 µm and a constant incident flux of 1.5 × 1012 photons s−1. For all derivative dose calculations two heavy atoms were added to each monomer of 175 amino acids. FcγRIII contains no methionines, but the absorbed dose for a selenomethionine derivative was calculated for illustrative purposes using the average frequency of methionine (1.8%; three per monomer). For the non-backsoaked platinum derivative a K2PtCl4 concentration of 250 mM was used. |