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Figure 3
(a) MXCuBE2 displays the photon flux incident on the sample in photons per second. The value is adjusted when changing the beam-defining aperture. This value and the beam size are given to EDNA to allow an estimation of the dose rate deposited on the crystal when calculating data-collection strategies. (b) Panel for EDNA characterization. Besides information on the data path and file name, users can adjust the complexity of the data collection (as in BEST; Bourenkov & Popov, 2010BB5), request a specific strategy for anomalous data collection by opting for `Optimize SAD' (Bourenkov & Popov, 2010BB5) and specify the crystal dimensions and space group, if already known. (c) Inverse beam is provided as an additional option for `standard data collection', in which the number of frames composing each subwedge (in this case ten) for the two data sets to be collected is defined. (d) Interleaved MAD workflow interface: up to four energies can be interleaved and frames can optionally be recorded in inverse-beam geometry. (e) Workflow interface for κ-angle reorientation: the anomalous strategy aims to align an evenfold axis parallel to ω. Possible κ strategies are described in Brockhauser et al. (2013BB7).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 72| Part 3| March 2016| Pages 413-420
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