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Figure 1
Initial ab initio structure determination of the 32-nucleotide dsRNA by direct methods using SIR2014 (PDB entry 5da6 ). (a) Data quality as indicated by Rmeas and the 〈I/σ(I)〉 signal-to-noise ratio. (b) The distribution of the final figure of merit (fFOM2) for the 194 phasing trials in the first experiment. (c) The weighted mean phase error (wMPE) versus resolution and the map correlation coefficient (mapCC) versus resolution for the winning phase set in (b) (Lunin & Woolfson, 1993BB36). The final refined model served as the source of the `true' phases. (d) Foexp(SIR2014 phases) electron-density map for dsRNA with the model from automated peak picking without knowledge of the RNA stereochemistry. The atom types are colored as follows: carbon, green; nitrogen, blue; oxygen, red; phosphorus, orange. The map was rendered with PyMOL.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 72| Part 4| April 2016| Pages 477-487
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