Figure 2
Cell sorting. Representative flow-cytometry scatter plots from (a) a non-infected cell sample (mock), which does not contain any crystals, and (b) Sf9 cells infected by a recombinant baculovirus coding for the CPV1 polyhedrin. Differences in the scattering pattern between the two populations allowed gating of the crystal-containing cell population (red gate, high SSC values). For comparison, a second population at lower values of SSC was also gated and collected. Representative optical microscopy images of each population are shown in the insets next to the corresponding gate. Scale bars, 50 µm. SSC, side light scattering; FSC, forward light scattering. |