Figure 7
Experimental phasing of in cellulo data. Superposition of the model autobuilt by Buccaneer (coloured teal) with the final refined model (coloured grey). The regions of the autobuilt model that are missing or incorrectly built are highlighted in red on the final model. The iodine and gold sites identified and refined by autoSHARP are represented as purple and gold spheres, respectively. (a) Close-up of a selected iodine site, with the bound iodine represented as a purple sphere. The corresponding isomorphous map contoured at 5σ is shown as a green mesh. The anomalous map did not show peaks above 5σ. The neighbouring residues of the protein are shown as sticks and labelled. (b) Close-up of a selected gold site, with the bound gold ion shown as a yellow sphere. The corresponding anomalous and isomorphous maps contoured at 5σ are shown as pink and green meshes, respectively. The neighbouring residues are shown as sticks and labelled. |