Structure of a quinolone-stabilized cleavage complex of topoisomerase IV from Klebsiella pneumoniae and comparison with a related Streptococcus pneumoniae complex

Veselkov, D.A.; Laponogov, I.; Pan, X.-S.; Selvarajah, J.; Skamrova, G.B.; Branstrom, A.; Narasimhan, J.; Prasad, J.V.N.V.; Fisher, L.M.; Sanderson, M.R.

doi:10.1107/S2059798316001212

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 6
Comparison of DNA cleavage by topoisomerase IV core ParE-ParC fusion proteins from K. pneumoniae (KP) and S. pneumoniae (SP) promoted by levofloxacin. Supercoiled plasmid pBR322 (400 ng) was incubated with topoisomerase IV proteins (400 ng) in the absence or presence of levofloxacin at the indicated concentrations. After 60 min incubation, samples were treated with SDS and proteinase K to remove proteins covalent bound to DNA, and the DNA products were examined by gel electrophoresis in 1% agarose. Lane A, supercoiled pBR322 DNA; N, L and S, nicked, linear and supercoiled pBR322, respectively.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 72| Part 4| April 2016| Pages 488-496

doi:10.1107/S2059798316001212

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