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Figure 4
Structures of free RBM39-UHM and of RBM39-UHM bound to U2AF2-ULM. (a) Intermolecular crystal contacts between symmetry-related RBM39-UHM molecules. The amino acids of the R-X-F motif are shown in red and hydrogen bonds as dotted lines; red spheres represent water molecules. Arrows indicate the mutation sites discussed in the text (Asn468Tyr and Thr510Tyr). (b) Crystal structure of the third RRM of RBM39-UHM bound to U2AF65-ULM. β-­Strands are colored blue, α-helices green and the ULM peptide yellow. The reciprocal tryptophan binding sites (Trp495 and Trp92) and the nearby residues proline (Pro95) and phenylalanine (Phe496) are shown as stick diagrams. The conserved R-X-F motif is indicated in red. (c) Structural alignment of the RBM39-UHM domain crystal structure (PDB entry 3s6e , chains A and B; both shown as cartoons) with the NMR solution structure (PDB entry 2lq5 ; shown in gray as a bundle of 20 conformers superimposed for minimal pairwise r.m.s.d.s relative to the mean coordinates; Table 3[link]). The labels A and B indicate the chains in the crystal structure.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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