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![[Figure 3]](mn5110fig3mag.jpg)
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Figure 3
αHis103(G10) in the α1:β1 interface supports the alkaline Bohr effect: T-state HuDeoxyHb α1:β1 interface (a, b), T-state HuDeoxyHb α2:β2 interface (c, d) and R-state EqCNmetHb α1:β1 interface (e, f). The α:β dimer interface indicates the position of αHis103, with the α subunits in wheat and the β subunits in gray (a, c, e). The electron and neutron density maps (b, d, f) display the H+/D+ states of αHis103(G10), which is doubly H+/D+-occupied in the T state with disrupted hydrogen-bond networks to βAsn108(G10), βGln127(H4) and βGln131(H8) (b, d) and singly H+/D+-occupied in the R state (f) with a rigid hydrogen-bond network. Here and in all figures, the nuclear density 2|Fo| − |Fc| maps are in blue, contoured at 1.0σ. OMIT nuclear density |Fo| − |Fc| maps are in red, contoured at 2.0σ. The exchanged D atoms are displayed in green, H atoms in white, N atoms in blue and O atoms in red; the black dashed lines indicate hydrogen bonds. |
![[Figure 3]](mn5110fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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