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![[Figure 1]](wa5110fig1mag.jpg)
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Figure 1
Linear gradient IEC–SAXS performed on BSA. (a) Standard ion-exchange chromatogram of BSA on the Uno Q-1R column, highlighting the locations of the three peaks. Orange line, percentage of buffer B; red line, resulting conductivity; violet line, absorbance at 280 nm. (b) IEC–SAXS chromatograms of BSA using a linear salt gradient. Top panel, UV absorbance (violet) and total scattering intensity (green). Middle and bottom panels, chromatograms of sample (red) and buffer (black) runs at q = 1 nm−1 (middle) and 4 nm−1 (bottom). (c) Difference between the scattering intensities measured at q = 4 nm−1 in the sample and buffer runs (blue symbols). The dotted orange line illustrates the salt gradient. (d) Comparison of the scattering ratio (0.11–0.5 nm−1 versus 1.5–2.5 nm−1) for background correction using a shift of 100 frames (cyan), 130 frames (blue) and 160 frames (green) between the sample and buffer runs. The dotted black line serves as a visual aid for a constant line. |
![[Figure 1]](wa5110fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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