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![[Figure 4]](wa5110fig4mag.jpg)
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Figure 4
Stepwise-gradient IEC–SAXS performed on BSA. (a) IEC–SAXS chromatograms of BSA using a stepwise salt gradient. Top panel, UV absorbance (violet) and total scattering intensity (green). Middle and bottom panels, chromatograms at 1 and 4 nm−1, respectively. (b) The buffer signal (mean scattering above 4.5 nm−1, blue) increases slowly during the elution of the peak (total scattering intensity, green) and can be modelled by an exponential decay (red) between the buffer before the peak (I) and after the peak (II). (c) Comparison of the scattering ratio (0.11–0.5 nm−1 versus 1.5–2.5 nm−1) for background correction using the buffer before the peak (I, cyan), after the peak (II, green) and the modelled buffer (blue). (d) Forward scattering (black), radius of gyration (green) and mass (red) based on the correlated volume for the background-corrected curves in the peak region. The grey area indicates the frames that were used for subsequent averaging; the arrows indicate the positions of the individual curves presented in (e) and (f). (e) Individual scattering curves and (f) Guinier plots at different positions throughout the chromatogram (curves are offset for clarity). The colour code and the positions in the chromatogram are as indicated in (d). |
![[Figure 4]](wa5110fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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