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Figure 5
Stepwise-gradient IEC–SAXS analysis of averaged BSA data. (a) Scattering curve based on averaging of the region indicated in grey in Fig. 4(d). (b) Guinier fit (light blue line) of the scattering curve shown in (a). Open symbols represent points that were not used for fitting. The lower panel shows the residuals. (c) Differences in scattering between the curve found by interpolating the buffer and a clearly under-subtracted curve (average of buffers from regions I and II, orange) and over-subtracted curve (buffer from region II, blue). (d) Fits to the monomeric crystal structure of BSA (PDB entry 3v03; Majorek et al., 2012BB29) eluted with a linear gradient (green) and a stepwise gradient (blue).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 72| Part 10| October 2016| Pages 1090-1099
https://doi.org/10.1107/S2059798316012833
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