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![[Figure 6]](wa5110fig6mag.jpg)
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Figure 6
IEC–SAXS of D5323–785. (a) Ion-exchange chromatogram of D5323–785 on the Uno Q-1R column with the three peaks indicated. Orange line, percentage of buffer B; red line, the resulting conductivity; violet line, absorbance at 280 nm. (b) IEC–SAXS chromatograms of D5323–785 using a stepwise salt gradient. Top panel, UV absorbance (violet) and total scattering intensity (green). Middle and bottom panels, chromatograms of sample (red) and buffer (black) runs at q = 1 nm−1 (middle) and 4 nm−1 (bottom). (c) Enlargement of the lower panel in (b): in the protein run (red) the signal at high q overshoots in comparison to the buffer run. |
![[Figure 6]](wa5110fig6.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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