Figure 7
(a) Average sample (violet) and buffer (black) curves for the D5323–785 protein, the resulting subtraction (red) and the fit for calculating the P(r) function (blue). (b) Guinier fit (black line) of the scattering curve shown in (a). Open symbols represent points that were not used for fitting. The lower panel shows the residuals. (c) Pair distance distribution function of the average curve, showing a single peak. (d) Differences in scattering between the curve based on the best-matching buffer and a clearly under-subtracted curve (preceding buffer step, orange) and over-subtracted curve (subsequent buffer step, blue). |