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![[Figure 4]](nj5264fig4mag.jpg)
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Figure 4
Generation of a truly apo structure by sequential dialysis against a solution containing glycerol and then only water. (a) Electron-density maps at 1.2 Å resolution for the apo structure after thorough dialysis, with lactose superimposed for comparison, showing only density for three water molecules. A 2m|Fo| − m|Fc| omit map contoured at 0.7σ is shown covering the lactose-binding site, His158 and Glu184 (grey mesh), as well as an |Fo| − m|Fc| map contoured at 3.5σ (green mesh). (b) Equivalent maps for the room-temperature apo X-ray structure (Saraboji et al., 2012  ) at 1.25 Å resolution contoured at the same levels. Residual electron density for parts of lactose can still be seen. (c) Equivalent electron-density maps at 0.86 Å resolution for the previous apo structure at 100 K. Full analysis of the apo structure (neutron and X-ray) will be presented elsewhere. |
![[Figure 4]](nj5264fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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