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Figure 4
Generation of a truly apo structure by sequential dialysis against a solution containing glycerol and then only water. (a) Electron-density maps at 1.2 Å resolution for the apo structure after thorough dialysis, with lactose superimposed for comparison, showing only density for three water molecules. A 2m|Fo| − m|Fc| omit map contoured at 0.7σ is shown covering the lactose-binding site, His158 and Glu184 (grey mesh), as well as an |Fo| − m|Fc| map contoured at 3.5σ (green mesh). (b) Equivalent maps for the room-temperature apo X-ray structure (Saraboji et al., 2012 ![]() |
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