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![[Figure 3]](jc5006fig3mag.jpg)
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Figure 3
CAD drawing of the coflow cell. The stainless steel assembly holds a custom 1 mm double-ended capillary which is sealed through O-ring compression by the top and bottom mounts. The outer O-rings provide a seal for in-vacuum (windowless) analysis if required. The top mount is open, allowing a needle to be positioned for submarine loading, and has connections for a continuous buffer supply, if needed, and a vacuum-assisted overflow port. The bottom provides the port for the total flow pump, which controls the total flow of buffer and sample through the capillary. At this stage of development the total flow was controlled by a VICI M50 displacement pump, and sample flow was driven by a Hamilton PSD-8 syringe pump. The arrow indicates the beam direction. |
![[Figure 3]](jc5006fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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