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Figure 9
Sensitivity/limits of detection of the coflow method. (a) Extended serial dilution series of glucose isomerase at 1.0 (grey), 0.50 (light blue), 0.25 (pink), 0.125 (dark blue), 0.0625 (yellow), 0.0313 (green), 0.0152 (red) and 0.0075 mg ml−1 (black). Data are binned in logarithmic q space. (b) Guinier fits of the data in (a) were conducted by linear regression of the data to the Guinier equation using SigmaPlot 13, which provides confidence intervals and standard errors of the slope of the fit, and hence the standard error of the radius of gyration and I(0). Data sets are offset by 0.5 units for clarity. (c) Observed radius of gyration, Rg (squares), and concentration-normalized intensity at zero q, I(0) (circles), are plotted versus concentration for serial dilution series of GI. The light grey line indicates the I(0) for 1 mg ml−1 GI (0.0455 cm−1). The dark grey line indicates the Rg for 1 mg ml−1 GI (32.8 Å). Uncertainties are ±2 standard errors of the slope of linear regression.

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