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Figure 3
Visualizing interactions with Glycoblocks. In the figure, the structure of a heavily glycosylated fungal glycosylhydrolase (PDB code 5fjj), reported by Agirre et al. (2016BB1). (a) View of the interactions of a high-mannose tree. The glycan connected to Asn323 is perhaps the only example of a three-dimensional structure of a complete high-mannose tree in the PDB. As the protein part has been coloured in rainbow style, it can immediately be seen that the glycan establishes hydrogen bonds across multiple domains and with other glycans which, in turn, interact with other parts of the protein. (b) Visualizing stacking interactions. The first GlcNAc sugar is linked in a flipped conformation to Asn443 due to the stacking interaction with Trp431 (W431 in the picture). These interactions are depicted in red. (c) Two-dimensional representation by Privateer. Dashed lines indicate an alpha link.

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