Figure 6
Individual adjustment of solvent concentration and soaking time is essential for successful soaking experiments. The working soaking conditions selected and used for 16 recent protein targets which were investigated as part of the XChem user program at the Diamond Light Source. The graphs show (a) the nominal final DMSO concentration used for fragment screening and (b) the soaking time selected for each project. A total of over 18 000 crystals were soaked in the course of these experiments. |