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![[Figure 5]](yt5100fig5mag.jpg)
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Figure 5
Crystal structure of Aae Hfq in the apo form, with head→tail stacking of hexameric rings. The apo form of Aae Hfq crystallized in space group P1 as a dodecameric assembly of hexamers stacked in a proximal→distal orientation in the lattice. Ribbon diagrams of the final, refined structure are shown here from perpendicular viewpoints. The proximal-exposed (PE) hexamer is colored blue and cyan, and subunits in the distal-exposed (DE) hexamer are colored alternatingly yellow and orange. Co-crystallizing molecules of MPD (gray C atoms) and Gnd (green C atoms) are shown in ball-and-stick representation, and Cl− ions are rendered as yellow spheres scaled to the van der Waals radius. Note that many of the Gnd cations and Cl− anions are coplanar, where they form a `salty' layer at the ring interface (this is most clearly seen in the transverse view). Contacts between hexamers are mediated by the N-termini of the DE hexamer (top) and the loop L2/strand β2 regions of the PE hexamer (bottom); the approximate location of one of the lateral rim RNA-binding sites is labeled on the DE ring. |
![[Figure 5]](yt5100fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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