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Figure 2
Non-phase-plate versus phase-plate data. A cryo-EM micrograph of FMDV taken at 1 µm defocus and its corresponding power spectrum (a, c) are compared with a micrograph and its corresponding power spectrum when aquired in focus and using the Volta phase plate (b, d). The images were taken with EPU at an equivalent total dose of around 30 e Å−2 at a pixel size of 1.06 Å per pixel using Krios 1 at eBIC. Images were collected on the Quantum K2 Summit detector in counting mode (∼6 electrons per pixel per second) with a 20 eV slit width. The FMDV virus particle can be clearly seen in the phase-plate image. The power spectra clearly show that the phase-plate image was in focus as there is no zero CTF present when compared with the 1 µm under-focus power spectrum. The samples were prepared by A. Kotecha, E. E. Fry, J. Seago and D. I. Stuart. The power spectra were calculated using Bsoft (Heymann et al., 2008BB11). The scale bar in (b) is 30 nm and the dashed rings in (c) and (d) are at 3.7 Å resolution.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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