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![[Figure 3]](ic5100fig3mag.jpg)
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Figure 3
Phase-plate tomography of the perforin pre-pore complex. The 0° image from the tomogram (a), the central ten z sections averaged from the reconstructed tomogram (b) and an enlarged 20-z-section average from the reconstructed tomogram (c) of perforin pre-pores bound to liposomes collected with the phase plate. The tomogram was collected on Krios II at eBIC at 1.7 Å per pixel using the Quantum K2 detector in counting mode (∼5 electrons per pixel per second) with a 20 eV slit width and a nominal defocus of 300 nm to avoid going over focus. Tilt images were collected from −45 to 45° in 3° increments, giving a total dose of around 60 e Å−2. The tomogram was collected using the FEI TOMO package and was processed with MotionCorr and IMOD (Li et al., 2013  ; Kremer et al., 1996). The scale bars in (a) and (b) are 100 nm and the scale bar in (c) is 10 nm. The grids were prepared by N. Lukoyanova. |
![[Figure 3]](ic5100fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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