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Figure 2
Purification and crystallization of YfeA. (a) SDS–PAGE gel showing the enrichment of YfeA during the various steps of purification. Molecular-weight standards are shown on the left (labeled in kDa). Lane 1, uninduced BL21 whole cells. Lane 2, BL21 whole cells induced with IPTG. Lane 3, lysate supernatant fraction following cell disruption with a French press. Lane 4, eluate from nickel-affinity chromatography. Lane 5, eluate from anion-exchange chromatography. Lane 6, gel-filtration peak fraction. (b) Superdex 200 10/300 GL gel-filtration chromatogram from YfeA purification. Fractions containing the peak from this chromatogram were concentrated, are represented in lane 6 in (a) and were used for crystallography. (c) Representative images of YfeA crystals.

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ISSN: 2059-7983
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