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Figure 5
Primary-structure alignment of YfeA ortholog SBPs. YfeA, designated as Yersinia, aligned with orthologs from other genera, including Gram-positive genera, with known three-dimensional structures: Salmonella enterica zinc-binding protein ZnuA (PDB entry 2xy4; Ilari et al., 2014BB23), Bacillus subtilis manganese-binding protein YcdH (PDB entry 2o1e; Northeast Structural Genomics Consortium, unpublished work), Treponema pallidum zinc-binding protein TroA (PDB entry 1toa; Lee et al., 1999BB26), Paracoccus denitrificans zinc-binding protein Pden1597 (PDB entry 4xrv; Handali et al., 2015BB21), Staphylococcus aureus manganese-binding protein MntC (PDB entry 4k3v; Gribenko et al., 2013BB20), Streptococcus pneumoniae manganese-binding protein PsaA (PDB entry 3ztt; McDevitt et al., 2011BB30), Listeria monocytogenes manganese-binding protein MntA (PDB entry 5i4k; Center for Structural Genomics of Infectious Diseases, unpublished work), Candidatus Liberibacter asiaticus manganese-binding protein CLas-ZnuA2 (PDB entry 4cl2; Sharma et al., 2015BB47) and Synechocystis sp. PCC 6803 manganese-binding protein MntC (PDB entry 1xvl; Rukhman et al., 2005BB43). Site 1 ligands from all orthologs are shown in yellow. Site 2 in YfeA is shown in dark blue. Tertiary, ancillary sites that co-load with site 2 are shown in purple, and sites that do not co-load with site 2 are shown in light blue.

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