Figure 6
EDS spectra of YfeA crystals. The EDS data show changes in the metal signal profiles according to cell-growth conditions. Data are represented as the mean ± 1σ. Asterisks indicate changes that are statistically significant. In M9 iron experiments, the change in iron had a calculated p value of 2.6 × 10−4 with α = 0.01. In M9 manganese experiments, the change in manganese had a calculated p value of 7.9 × 10−6 with α = 0.01. Zinc remains the dominant substrate in native, untagged YfeA that is overexpressed in the context of YfeBCDE and purified from the periplasm. |