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Figure 9
Fractionation of E. coli cells overexpressing the Yfe locus by an endogenous promoter. (a) SDS–PAGE gel showing expression of Yfe locus components and localization of YfeA in the E. coli BL21-CodonPlus (DE3)-RIPL periplasm. Molecular-weight standards are shown on the left (labeled in kDa). Lane WC, whole cells. Lane OM, outer membrane fraction. Lane PP, periplasm fraction. Lane IM, inner membrane fraction. Lane CY, cytoplasm fraction. (b) Mass-spectrometric sequence coverage of YfeA, shown in green, in the whole-cell and periplasm fractions. (c) Mass spectrum of YfeB in the whole-cell fraction.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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