Figure 5
Overview of the solutions that were not well suited for HPC or cooling from Fig. 4. Screening solutions from the respective screens that failed to be cooled to amorphous ice are listed. Wells marked in yellow can be used for HPC if the PEG concentration is raised by 1–2%(w/v). Conditions marked in orange can be cooled by HPC if the PEG concentration is further increased by up to 8%(w/v). The remaining five of the 288 conditions need conventional treatment with additional cryoprotectants. |