Figure 6
In vitro MC R354 iron-precipitation assay. (a) Magnetic particles prepared in vitro by magnetite precipitation with MBP-MamC-MIC, MC R354 and buffer. Scale bars represent 200 nm. The protein concentration was 10 µg ml−1. (b) Selected-area electron diffraction (SAED) of the crystals shown in (a). (c) Size distribution of magnetite particles prepared with different proteins at a concentration of 10 µg ml−1 or in a protein-free experiment. The red line refers to the maximum size achieved by 90% of the particle population in the control experiment. The statistical significance of differences from MBP-MamC-MIC was tested using an ANOVA test (P < 6.43 × 10−32). |