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![[Figure 4]](jt5022fig4mag.jpg)
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Figure 4
Crystal structure of GH18B and chitooligosaccharide-complexed GH18B. (a) Cartoon representation of the overall structure of GH18B. The TIM barrel and CID are shown in white and gold, respectively. The aromatic residues that line the substrate-binding groove are shown in blue and the catalytic residues are shown in red. (b) The intermolecular interactions between the amino-acid residues in the substrate-binding groove of GH18B-E647L and (GlcNAc)5. Hydrogen bonds are shown as black dashed lines. (c) The differences in the binding modes of cleaved (GlcNAc)3 (in yellow) in wild-type GH18B and intact (GlcNAc)5 (in green) in GH18A-E647L. The C2 acetamido groups with different conformations are circled. (d) Shrinkage of the substrate-binding groove of GH18B after (GlcNAc)5 binding (unliganded GH18B-E647L, white; GH18B-E647L–(GlcNAc)5, blue). |
![[Figure 4]](jt5022fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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